Gene Technology & Protein Expression

Subject 521-321 (2008)

Note: This is an archived Handbook entry from 2008.Search for this in the current handbook

Credit Points: 12.500
Level: Undergraduate
Dates & Locations:

This subject has the following teaching availabilities in 2008:

Semester 1, - Taught on campus.
Pre-teaching Period Start not applicable
Teaching Period not applicable
Assessment Period End not applicable
Last date to Self-Enrol not applicable
Census Date not applicable
Last date to Withdraw without fail not applicable

Timetable can be viewed here. For information about these dates, click here.
Time Commitment: Contact Hours: 48 hours practical work (four hours a week) plus 12 hours of lectures (one per week)
Total Time Commitment: 120 hours
Prerequisites: Biochemistry 521-211, 521-212 and 521-220.BBiomedSc students: 521-213 and 536-250.
Corequisites: None
Recommended Background Knowledge: None
Non Allowed Subjects: None
Core Participation Requirements: It is University policy to take all reasonable steps to minimise the impact of disability upon academic study and reasonable steps will be made to enhance a student’s participation in the University’s programs. Students who feel their disability may impact upon their active and safe participation in a subject are encouraged to discuss this with the relevant subject coordinator and the Disability Liaison Unit.


Mrs B Bencina; A/Prof H-C Cheng
Subject Overview:

To participate in the rapidly expanding field of genome research, it is necessary to have an understanding of the techniques for handling both DNA and recombinant proteins. This subject aims to provide both. Students will receive training in the basic laboratory skills for manipulation of DNA and proteins and apply these skills to a number of biotechnological investigations.

Areas covered include the use of recombinant DNA for the investigation of gene function and the use of bacterial expression systems for the production and analysis of recombinant proteins. Specific experiments will deal with PCR, plasmid purification, DNA cloning and sequencing and bioinformatics.

In addition to these specific skills, students will develop an appreciation for the current scientific literature and acquire problem-solving skills through collaborative work.

Students will learn how to maintain a laboratory notebook containing a detailed record of the experiments carried out and prepare written reports describing these experiments.

The experimental work will be organised into elective streams, one of which will involve an opportunity to undertake relevant project work in one of the department's research laboratories (a quota will apply for project work).

The practical unit will be supported by a lecture series addressing current advances in these technologies. Topics include cDNA cloning and sequencing, sequence databases and analysis; recombinant expression systems; protein purification; and immunochemistry.

Assessment: Ongoing assessment of laboratory skills and practical management of the experimental program throughout the semester (30%); a 2-hour written examination in the examination period (30%).Laboratory course: a written research report of up to 2000 words and a laboratory notebook submitted during the semester (40%).Project work: maintenance of a laboratory notebook throughout the semester and a written research report of up to 2500 words to be submitted by the end of the semester (40%).
Prescribed Texts: None
Breadth Options: This subject is a level 2 or level 3 subject and is not available to new generation degree students as a breadth option in 2008.
This subject or an equivalent will be available as breadth in the future.
Breadth subjects are currently being developed and these existing subject details can be used as guide to the type of options that might be available.
2009 subjects to be offered as breadth will be finalised before re-enrolment for 2009 starts in early October.
Fees Information: Subject EFTSL, Level, Discipline & Census Date

Students enrolled in the BSc (pre-2008 BSc), BASc or a combined BSc course will receive science credit for the completion of this subject.

Before the commencement of the semester, students must advise the Department of Biochemistry and Molecular Biology of their order of preference for the alternative practical sessions and the other subjects they will be taking. (See subject web site for details).

Related Course(s): Bachelor of Arts and Bachelor of Science
Bachelor of Arts and Sciences
Bachelor of Biomedical Science
Bachelor of Science
Graduate Diploma in Biotechnology

Download PDF version.