Advanced Techniques in Molecular Science

This subject has the following teaching availabilities in 2016:

An enrolment quota of 90 students (30 students per practical class) per semester applies to this subject.

For detailed information on the quota subject application process, refer to the Quota Subject link on the MDHS Student Centre website: http://sc.mdhs.unimelb.edu.au/quota-subjects

84 contact hours with an estimated total time commitment of 170 hours.

BSc students

Note: Both a Biochemistry and Molecular Biology lecture subject and practical subject are required

Before 2009:

Biochemistry & Molecular Biology Part A (521-211)
Biochemistry & Molecular Biology Part B (521-212)
Techniques in Molecular Science (521-220)

OR

Techniques in Protein and Gene Technology (521-220)

2009 and subsequently:

Note that the pre-2009 subject “Biochemistry & Molecular Biology Part A” and the 2009 subject “BCMB20002 Biochemistry & Molecular Biology” are not identical despite sharing a similar subject title.

Only the subject

offered in 2009 and beyond acts as a prerequisite for the lecture subject (replacing both the pre-2009 Biochemistry and Molecular Biology Part A and Biochemistry and Molecular Biology Part B).

BBiomedicine students

All Students: Other combinations of subjects that provide a similar background may be considered by the coordinator.

None

BSc students

BCMB20003 Biochemical Regulation of Cell Function is recommended.

BBiomedicine Students

BCMB20003 Biochemical Regulation of Cell Function is strongly recommended.

Students cannot enrol in and gain credit for this subject if credit was obtained for pre-2009 subjects Gene Technology & Protein Expression (521-321) or Protein Biochemistry & Proteomics (521-322).

For the purposes of considering request for Reasonable Adjustments under the Disability Standards for Education (Cwth 2005), and Student Support and Engagement Policy, academic requirements for this subject are articulated in the Subject Overview, Learning Outcomes, Assessment and Generic Skills sections of this entry.

It is University policy to take all reasonable steps to minimise the impact of disability upon academic study, and reasonable adjustments will be made to enhance a student's participation in the University's programs. Students who feel their disability may impact on meeting the requirements of this subject are encouraged to discuss this matter with a Faculty Student Adviser and Student Equity and Disability Support: http://services.unimelb.edu.au/disability

To participate in the rapidly expanding fields of genome research and protein structure-function analysis it is necessary to have an understanding of the techniques used in these areas.

This subject provides practical training in the technologies of molecular biology, protein expression and molecular cell biology. Analysis of the data derived from these techniques is also integral to these studies.

Areas covered include the use of recombinant DNA for the investigation of gene function, the use of bacterial expression systems for the production and analysis of recombinant proteins; mass spectrometry to identify proteins and the use of fluorescently labelled proteins to understand sub-cellular targetting in mammalian cells.

Specific experiments will deal with DNA cloning and sequencing, enzyme mutagenesis and expression, the identification of proteins in mammalian sera and using fluorescent microscopy to localise subcellular localisation of proteins in mammalian cells.

Students will learn how to maintain a laboratory notebook to record their experiments and how to compose a scientific report. In addition, students will develop an appreciation for the current scientific literature and collaborate in student presentations.

The experimental work is supported by a lecture series providing an overview of technologies used in class and in research.

• to provide practical experience in a variety of techniques used in biochemistry and molecular biology.
• to give instruction in the correct methods for keeping scientific records and writing scientific reports.
• to provide experience in simple experimental design and problem solving.
• to extend students' knowledge of the use of bioinformatics in the analysis of DNA and protein sequence data and in data derived by mass spectrometry.
• to assist students in the evaluation of scientific literature and to develop skills in the presentation of scientific data in oral and written formats.

Ongoing assessment of laboratory skills throughout the semester (45%) divided between:

• practical management of the experimental program,
• maintenance of a laboratory notebook including short summaries and brief reports of practical results and

Written research report based on the first 4 weeks of the semester, 2500 words (15%)

Group presentation of a scientific paper: a 15 min preserntation by a group of 5 students with guidance and assistance from an academic mentor, due at the end of semester (15%)

Two database tutorials: DNA and Literature Bioinformatics due in week 4, and Mass Spectrometry (MS) Bioinformatics due in week 11 (5%)

2-hour written examination in the examination period at the end of semester (20%)

Attendance is compulsory. Students who miss more than 20% of the practical classes will not be eligible for final assessment.

None

Keith Wilson and John Walker, Principles and Techniques of Biochemistry and Molecular Biology (2010, 7th Ed) Cambridge University Press

This subject is not available as a breadth subject.

On completion of this subject, students should have developed the following generic skills:

• Hands-on experience in a variety of techniques, generating results for analysis.
• Design and execution of simple experiments.
• Analysis of experimental data using spreadsheets and bioinformatics resources.
• The ability to keep complete and accurate records of experimental results and to use these records to prepare a scientific report.
• Evaluation and presentation of scientific literature to an audience.
• The ability to interpret scientific literature and interpret data from electronic databases.
• The capacity to integrate knowledge across disciplines.
• The ability to comprehend a question, evaluate the relevant information and communicate an answer.

This subject is available for science credit to students enrolled in the BSc and in BBiomed.

Students must enrol for one of the available laboratory days via the student portal before the start of the semester.

Be aware that each day may have limited places and in general allocations to practical classes will be made on a first come-first served basis.

Students undertaking this subject will be expected to have regular access to an internet-enabled computer.